normal human renal cortical epithelial cells cc-2554 Search Results


94
ATCC astrocytes
Astrocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC renal cortical epithelial cells
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Renal Cortical Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ScienCell epicm
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Epicm, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ScienCell epithelial cell growth supplement (epicgs
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Epithelial Cell Growth Supplement (Epicgs, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
ATCC hct 116
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Hct 116, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ScienCell human pulmonary artery fibroblasts
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Human Pulmonary Artery Fibroblasts, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc plenticrisprv2 addgene
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Plenticrisprv2 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc plentiguide puro addgene
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Plentiguide Puro Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
BioWhittaker Molecular Applications human renal proximal tubule epithelial cells hrpt; cc2553
Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal <t>epithelial</t> cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).
Human Renal Proximal Tubule Epithelial Cells Hrpt; Cc2553, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal epithelial cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).

Journal: Cell Reports Medicine

Article Title: Preclinical development of a chimeric antigen receptor T cell therapy targeting FGFR4 in rhabdomyosarcoma

doi: 10.1016/j.xcrm.2023.101212

Figure Lengend Snippet: Low or absence of cell surface FGFR4 in primary human cells does not induce cytokine release when cocultured with 3A11 CAR T cells (A) Representative flow cytometry plots show low or absence of FGFR4 cell surface expression on human cardiomyocytes, renal epithelial cells, renal cortical epithelial cells, renal proximal epithelial cells, HEK293, cholangiocytes, and hepatocytes pooled from 10 individuals using 3A11 scFvFc. MFI of 3A11 scFvFc or huIgG1 isotype control staining on these primary cells is shown in the top right table. Relative FGFR4 expression levels on these primary cells compared with RH30 cells are calculated as R e l a t i v e e x p r e s s i o n = [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] P r i m a r y c e l l [ M F I ( s c F v F c ) − M F I ( h u I g G i s o t y p e ) ] R H 30 . (B) Log 2 ratio of cytokine (IFN-γ, IL-2, and TNF-α) release in the supernatant, by 3A11 CAR T cells cocultured with primary cells, as indicated, in their respective media, compared with the RMS cell RH30. Cell line 7250 serves as a FGFR4 − control. Values represent n = 3 independent experiments with n = 3 individual donors. Values represent mean ± SEM (error bars).

Article Snippet: Human cardiomyocytes (Cat# 36044-15), cholangiocytes (Cat# 36755-12), and pancreatic epithelial primary cells (Cat# 36099-25) were obtained from Cellprogen; human coronary artery smooth muscle cells (CASMC, Cat# CC-2583), small airway epithelial cells (SAEC, Cat# CC-2547), bronchial/tracheal epithelial cells (NHBE, Cat# CC-2541), human renal epithelial cells (HRE, Cat# CC-2556), renal cortical epithelial cells (HREC, Cat# CC-2554), renal proximal tubule epithelial cells (RPTEC, Cat# CC-2553), hepatocytes (Cat# HUCPG), and 10 Donors pooled hepatocytes (Cat# HUCS10P) were purchased from Lonza; HEK 293 cells (Cat# CRL-1573) was purchased from ATCC.

Techniques: Flow Cytometry, Expressing, Control, Staining

Journal: Cell Reports Medicine

Article Title: Preclinical development of a chimeric antigen receptor T cell therapy targeting FGFR4 in rhabdomyosarcoma

doi: 10.1016/j.xcrm.2023.101212

Figure Lengend Snippet:

Article Snippet: Human cardiomyocytes (Cat# 36044-15), cholangiocytes (Cat# 36755-12), and pancreatic epithelial primary cells (Cat# 36099-25) were obtained from Cellprogen; human coronary artery smooth muscle cells (CASMC, Cat# CC-2583), small airway epithelial cells (SAEC, Cat# CC-2547), bronchial/tracheal epithelial cells (NHBE, Cat# CC-2541), human renal epithelial cells (HRE, Cat# CC-2556), renal cortical epithelial cells (HREC, Cat# CC-2554), renal proximal tubule epithelial cells (RPTEC, Cat# CC-2553), hepatocytes (Cat# HUCPG), and 10 Donors pooled hepatocytes (Cat# HUCS10P) were purchased from Lonza; HEK 293 cells (Cat# CRL-1573) was purchased from ATCC.

Techniques: Purification, Control, Recombinant, Labeling, Virus, Microarray, Cytokine Assay, Transfection, Chromatin Immunoprecipitation, ChIP-sequencing, Plasmid Preparation, Software, Imaging